The overall objective of the project is to determine whether model organisms with genetically sensitized signal transduction pathways can be used as predictors of mammalian developmental toxicity.
– Evaluate the effect of model developmental toxicants on the morphological development of a limited number of strains of Drosophila, C. elegans, and zebrafish that are sensitized for specific signal transduction pathways. Results using the sensitized strains will be compared either with wild-type organisms or with organisms heterozygous for the sensitizing mutations.
– Evaluate the effect of model developmental toxicants on the biochemical function of key signal transduction pathways to further support the biological relevance, at a molecular level, for the prediction of mammalian developmental toxicity.
– Determine whether human-relevant metabolizing systems can be incorporated into the sensitized screens.
Research project summary:
Evaluation of Signal Transduction Pathways in Model Organisms as Critical Mediators of Developmental Toxicity by G. Daston, J. Naciff, G. Overmann, Y. Shan, X. Wang, S. Patel and Jing Lu