Measurement of ocular irritancy is a necessary step in the safety evaluation of both industrial and consumer products. Assessment of the acute eye irritation potential is therefore part of the international regulatory requirements for testing of chemicals.
This project will:
- Identify a set of 80 chemicals based on reliable existing in vivo and in vitro data on eye irritation.
- Assess the reliability of 8 in vitro test systems: 1) BCOP (Bovine Corneal Opacity and Permeability and 2) BCOP-LLBO (BCOP-laser light-based opacitometer), 3) ICE (Isolated Chicken Eye), 4) EpiOcular-EIT (EpiOcular Eye Irritation Test) 5) and EpiOcular-ET50, 6) SkinEthicTM HCE (Human Corneal Epithelial), 7) STE (Short Term Exposure), and 8) SMI (Slug Mucosal Irritation) to define eye irritation potential.
- Define applicability domains, strengths and limitations of the in vitro test systems.
- Proposal for a tiered-testing strategy for eye irritation assessment.
Verstraelen S., Adriaens E., Hollanders K., Boonen F., Alepee N., Kandarova H., Drzewiecka A., Fochtman P., Gruszka K., Willoughby J. A., Guest R., Maglennon G., Schofield J. and Van Rompay A.R., "Is BCOP included in an integrated testing strategy for eye irritation assessment?", published at EUROTOX event 5-7/09/2016.
Kandarova H., Van Rompay A., Adriaens E., Alepee N., Drzewiecka A., Fochtman P., Gruszka K., Guest R., Maglennon G., Schofield J., Willoughby J. A. and Verstraelen S. , "CON4EI: EpiOcular Eye Irritation Test (EIT)", published at EUROTOX event 5-7/09/2016