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EMSG34.4-SYNG: Evaluation of the weanling and testosterone-stimulateed weanling rat as an alternative to the Hershberger castrated rat in the detection of coded androgens and antiandrogens

Principal Investigator

Dr.Jenny Odum
Central Toxicology Laboratory
Alderley Park
Macclesfield, SK10 4TJ
Cheshire, UK
Tel: + 44 (0)1625 516182
Fax: + 44 (0)1625 590996


Dr. Remi Bars, Bayer CropScience, FR
Dr. Alexius Freyberger, Bayer Healthcare, FR
Dr rer. nat. Christian Boegi, BASF Aktiengesellschaft, DE


Growing concerns regarding the possibility that environmental contaminants could mimic estrogens and androgens and hence disrupt endocrine systems have led to the evaluation of test systems to detect such contaminants. One system, the weanling rat assay, has been suggested as a possible non-invasive replacement for the Hershberger castrated rat test for androgens and antiandrogens. The weanling assay, measuring the growth stimulation of sex accessory tissues (SAT) to monitor for androgenic activity and the inhibition of testosterone-stimulated SAT weight increases by antiandrogens, has been used to investigate dose responses to both androgens and antiandrogens. The current proposed investigation involves two studies using the weanling assay to test the response to a series of defined coded compounds. One study will monitor androgenic activity; the other will monitor antiandrogenic activity. Thus, the assay’s reproducibility and robustness will be tested in different laboratories, using different personnel, with a series of carefully controlled and defined coded compounds.

Timeline: March 2006 > November 2006

LRI funding: €48,063


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