Principal Investigator
Dr. Tom Hutchinson
AstraZeneca
Brixham Environmental Laboratory
Freshwater Quarry
UK – TQ5 8BA Brixham
tom.hutchinson@brixham.astrazeneca.com
Tel: +44 1803 88 42 72
Fax: +44 1803 88 29 74
Collaborators
Participating Laboratories:
AstraZeneca, UK
Brunel University, UK
Schering, Germany
Shell, UK
University of Konstanz, Germany
Description
An in vivo screening test for oestrogenicity was developed jointly by three laboratories by measuring vitellogenin in fathead minnows after up to 21 days exposure. This method will be further refined by determination of (anti-)androgens and aromatase inhibitors in the fish so as to widen the scope to other mode of action. This method will soon be validated with a further set of test compounds by Cefic/LRI-EMSG funded laboratories. The results of this ongoing work have been successful in helping the OECD in developing future fish test guidelines for assessing endocrine modulators.
Related Publications
Publications:
GH Panter, TH Hutchinson, KS Hurd, A Sherren, RD Stanley, CR Tyler. Successful detection of (anti-)androgenic and aromatase inhibitors in pre-spawning adult fathead minnows (Pimephales promelas) using easily measured endpoints of sexual development. Aquat Toxicol. 2004 Oct 18;70(1):11-21.
GH Panter, TH Hutchinson, R Länge, CM Lye, JP Sumpter, M Zerulla, CR Tyler. Utility of a juvenile fathead minnow screening assay for detecting (anti-)estrogenic substances. Environ Toxicol Chem. 2002 Feb;21(2):319-26.
