This project aims to develop a non-animal testing strategy to reduce the need for fish early life-stage toxicity tests (FELS) for the assessment of chronic toxicity of chemicals to fish, using the adverse outcome pathway (AOP) framework as a guidance for assay development.
The aim of ECO20.2 is to take advantage of the existing momentum in AOP development to consolidate our previous efforts into practical tools for risk assessment.
We selected the two most promising AOPs developed in ECO20, the thyroid AOP and the narcosis AOP.
- We will apply the alternative assays which were developed during ECO20 on a set of ±25 compounds per AOP, and predict acute and chronic toxicity based on the assay results.
- Subsequently, we will perform a selected set of acute (FET) and chronic (FELS) toxicity experiments in order to validate the predictions. We envisage performing such validation experiments for a total of 5-7 compounds.
The results of ECO20.2 will be used as a proof-of-concept of using AOPs for assay development for screening and prioritization of chemicals.
Stinckens, E., Vergauwen, L., Cavallin, JE., Blackwell, BR., Witters, H., Blust, R., Ankley, GT., Volz, DC., Villeneuve, DL., Knapen D. (2017). Development of an alternative testing strategy for the fish early life-stage test using the AOP network “thyroperoxidase and deiodinase inhibition leading to impaired swim bladder inflation”. SETAC Europe 27th Annual Meeting, Brussels, Belgium. May 7-11, 2017.
Stinckens, E., Vergauwen, L., Cavallin, JE., Schroeder, AL., Blackwell, BR., Witters, H., Blust, R., Ankley, GT., Villeneuve, DL., Knapen D. (2017). Cross-species assay validation using the AOP “deiodinase inhibition leading to impaired posterior chamber inflation”. SETAC Europe 27th Annual Meeting, Brussels, Belgium. May 7-11, 2017.